Optimization of the biosynthesis of polyhydroxyalkanoates by native Guatemalan bacteria using whey as a substrate
DOI:
https://doi.org/10.36829/63CTS.v11i1.1598Keywords:
Biopolymers, biodegradable plastics, waste reuseAbstract
Polyhydroxyalkanoates (PHA) are biodegradable polymers that could replace petroleum-based plastics due to their properties, such as biodegradability, biocompatibility, safety, and thermoplasticity. Despite their benefits, the large-scale utilization of PHA is limited by deficiencies in the production processes, especially regarding the costs associated with raw materials. It has been estimated that improvements in the PHA production process through the utilization of waste residues such as whey or agro-industrial waste can significantly reduce production costs by up to 50%. In this research, the biotechnological potential of native bacteria for PHA production using whey as a substrate was evaluated. The chloroform-hypochlorite methodology was employed for biopolymer extraction, and the amount of PHA was determined by spectrophotometry. It was found that out of the 40 evaluated strains, 28 were capable of utilizing whey as a substrate. Additionally, it was determined that the optimal fermentation conditions were a temperature of 37 °C, pH of 7, and 150 RPM. The fermentation conditions tested in this research could be applied on a larger scale for PHA production, primarily due to the similarity in biopolymer production yield obtained compared to chemically defined media and the cost reduction that the utilization of dairy industry by-products would provide.
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