Identificación de microorganismos del género Phytophthora asociados a especies de Quercus sp. y Pinus sp., en los departamentos de Guatemala y Sacatepéquez

Jose Humberto Calderon Diaz; Maria del Carmen Santos Bravo

doi:10.36829/63CTS.v2i1.59

Authors

Jose Humberto Calderon Diaz Instituto de Investigaciones Agronómicas y Ambientales, Facultad de Agronomía, Universidad de San Carlos de Guatemala, Guatemala
Maria del Carmen Santos Bravo Instituto de Investigaciones Agronómicas y Ambientales, Facultad de Agronomía, Universidad de San Carlos de Guatemala, Guatemala

DOI:

https://doi.org/10.36829/63CTS.v2i1.59

Keywords:

Phytophthora, Pinus, Quercus, incidence, severity, pathogenicity

Abstract

The mean purpose of this research was to identify microorganism belonging to Phytophthora genera which are affecting mixed natural forests and nurseries of Pinus sp. and Quercus sp. These species have economic and social impact in provinces such as Guatemala and Sacatepéquez. Soil and vegetal tissue were used to isolate Phytophthora from natural forest of Guatemala, 45 Pinus sp. and 10 Quercus sp. were sampled and from nurseries 88 Pinus sp. and 11 Quercus sp. From Sacatepéquez province, from natural forest system were sampled 48 Pinus sp. and 15 Quercus sp. From nurseries were sampled 58 Pinus sp. and 25 Quercus sp. After processing the samples from soil and roots 13 were found positive to Phytophthora sp. in Pinus maximinoi, 10 from Guatemala and three from Sacatepéquez provinces. The culture of Phytophthora sp. on PDA produced two colonies without define form and five stoloniferous, five semipetaloid, one stelade type colonies. VP16 isolate was inoculated in five species of pine for pathogenicity test, causing high percentages of incidence and severity on Pinus caribaea, P. oocarpa, P. pseudostrobus and P. maximinoi and low rates of incidence and severity on Pinus tecunumanii.

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References

Álvarez, C. (1988). Tamaño de muestra: Procedi- mientos usuales para su determinación. (Tesis de maestría). Colegio de Post-graduados, Cha- pingo, México.

Alvarez, G. A. (2010). Detección y caracterización de especies de Oomycetes asociados a cultivos de exportación en la región central de Guatemala. (FODECYT No. 2007.01). Guatemala: Consejo Nacional de Ciencia y Tecnología, Universidad de San Carlos de Guatemala, Facultad de Agro- nomía.

Brasier, C., & Jung, T. (2003). Progress in understand- ing Phytophthora diseases of trees in Europe. En J. McComb, G. Hardy & I. Tommerup (Eds.), Phytophthora in forests and natural ecosystems. Proceedings of the Second International Meeting of IUFRO Working Party 7.02.091 (pp. 4-18). Recuperado de http://forestphytophthoras.org/sites/ default/files/proceedings/IUFRO%25202001.pdf

Erwin, D. C., & Ribeiro, O. K. (1996). Phytophthora diseases worldwide. St. Paul, Minnesota: Ameri- can Phytopathological Society.

French, E. R., & Hebert, T. T. (1980). Métodos de investigación fitopatológica (Serie Libros Mate- riales Educativos no. 43). San José, Costa Rica: Instituto Interamericano de Ciencias Agrícolas.

González, V., Velarde, E., & Abad, J. (1979). Chupadera fungosa de Pinus spp. en un vivero de Cajamarca (UNTC). Revista Forestal del Perú, 9(2), 1-6.

Hansen, E. M. (2000). Phytophthora in forests of the Americas. In E. Hansen & W. Sutton (Eds.), Phytophthora Diseases of Forest Trees. Procee- dings of 1st International Meeting on Phytophtho- ras in Forest and Wildland Ecosystems. Interna- tional Union of Forestry Research Organizations Working Party 7.02.09. Grants Pass Oregon, August 29 - 3 September 1999 (pp. 23-27). Cor- vallis, Oregon, USA: Forest Research Laboratory, Oregon State University.

Jeffers, N. S., & Martin, J. B. (1986). Comparison of two media selective for Phytophthora and Pythium species. Plant Disease, 70(11), 1038-1043. https://doi.org/10.1094/PD-70-1038

Jung, T., Blaschke, H., & Oßwald, W. (2000). Involvement of Phytophthora species in Central European oak decline and the effect of site factors on the disease. Plant Pathology, 49, 706-718. https://doi.org/10.1046/j.1365-3059.2000.00521.x

Jung, T., Cooke, D. E., Blaschke, H., Duncan, J. M., & Oßwald, W. (1999). Phytophthora quercina sp. nov., causing root rot of European oaks. Mycological Research, 103, 785-98. https://doi.org/10.1017/S0953756298007734

Jung, T., Hansen, E. M., Winton, L., Oßwald, W., & Delatour, C. (2002). Three new species of Phytophthora from European oak forests. Mycological Research, 106 , 397-411. https://doi.org/10.1017/S0953756202005622

Jung, T., Nechwatal, J., Cooke, D. E. L., Hartmann, G., Blaschke, M., Oßwald, W., Duncan, J. M., & Delatour, C. (2003). Phytophthora pseudosyringae sp. nov., a new species causing root and collar rot of deciduous tree species in Europe. Mycological Research, 107, 772-789. https://doi.org/10.1017/S0953756203008074

Newhook, F. J., Waterhouse, G. M., & Stamps, D. J. (1978). Tabular key to the species of Phytophthora de Bary. (Mycological Papers, no.143). Kew, Surrey: Commonwealth Mycological Institute.

Otrosina, W., & Marx, D. (1975). Populations of Phytophthora cinnamomi and Pythium spp. Un- der shortleaf and loblolly pines in littleleaf disease site. Phytopathology, 65, 1224-1229. https://doi.org/10.1094/Phyto-65-1224

Sánchez, M. E., Sánchez, J. E., Navarro, R. M., Fer- nández, P., & Trapero, A. (2003). Incidencia de la podredumbre radical causada por Phytophthora cinnamomi en masas de Quercus en Andalucía. Boletín Sanidad Vegetal, 29(1), 87-108. Recuperado de http://www.magrama.gob.es/ministerio/ pags/biblioteca/plagas/BSVP-29-01-087-108.pdf

Santos, M. C. (2011a). Bioprospección y diversidad de Phytophthora y Phytium asociados a especies forestales de importancia económica en la región central de Guatemala, en fase de viveros. (FODE- CYT No. 2009.76 ). Guatemala: Consejo Nacio- nal de Ciencia y Tecnología, Universidad de San Carlos de Guatemala, Facultad de Agronomía.

Santos, M. C. (2011b). Bioprospección de Phytophthora sp. asociado a especies forestales de importancia económica en fase de vivero en la región central de Guatemala (Tesis licenciatura). Universidad de San Carlos de Guatemala, Facultad de Agronomía, Guatemala.

Sechi, C., Seddaiu, S., Linaldeddu, B. T., Franceschini, A., & Scanu, B. (2014). Dieback and mortality of Pinus radiata trees in Italy associated with Phytophthora cryptogea. Plant Disease, 98, 159. https://doi.org/10.1094/PDIS-05-13-0572-PDN

Singleton, L., Mihail, J., & Rush, C. (Eds.). (1992). Methods for iesearch on soilborne Phytopathogenic Fungi. St. Paul, Minnesota: American Phytopathological Society Press.