Cristalogénesis biológica y cristalografía en la elucidación de la estructura tridimensional de las proteínas. Revisión narrativa

Omar E. Vélasquez-González; Luis Amézquita-Morataya

doi:10.36829/63CTS.v9i2.1032

Authors

Omar E. Vélasquez-González Instituto de Química, Universidad Nacional Autónoma de México y Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala
Luis Amézquita-Morataya Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala https://orcid.org/0000-0001-5526-3658

DOI:

https://doi.org/10.36829/63CTS.v9i2.1032

Keywords:

Protein tridimensional structure, biological crystallogenesis, crystallography, X – ray diffraction, drug design

Abstract

Obtaining three-dimensional structural information about a protein is of utmost importance in various fields such as functional biochemistry, materials science, or biomedical sciences. Even though single-crystal X-ray diffraction is currently the gold standard for this purpose, growing said single crystal is still a bottleneck from a practical viewpoint and not fully understood from a theoretical point of view. In this article, we review, from a protein structure perspective, the way X-rays provide structural information and the physicochemical conditions that promote the formation of an adequate crystal for these experiments.

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Author Biography

Omar E. Vélasquez-González, Instituto de Química, Universidad Nacional Autónoma de México y Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala

Licenciado en Química USAC, Maestro en Ciencias Químicas UNAM, Profesor Titular Departamento de Fisicoquímica, Facultad de Farmacia, USAC.

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