Identificación de nuevas fuentes de resistencia a antracnosis en el germoplasma de frijol arbustivo del altiplano de Guatemala

Carlos Rául Maldonado-Mota; María Gabriela Tobar Piñón; Julio Cesar Villatoro Mérida

doi:10.36829/63CTS.v10i1.1003

Authors

Carlos Rául Maldonado-Mota Instituto de Ciencia y Tecnología Agrícolas https://orcid.org/0000-0002-1048-4518
María Gabriela Tobar Piñón Instituto de Ciencia y Tecnología Agrícolas https://orcid.org/0000-0002-1442-5224
Julio Cesar Villatoro Mérida Instituto de Ciencia y Tecnología Agrícolas

DOI:

https://doi.org/10.36829/63CTS.v10i1.1003

Keywords:

C. lindemuthianum, dry bean, Phaseolus vulgaris L, genetic resistance

Abstract

Dry bean (Phaseolus vulgaris L.) is the second main crop in Guatemala and is the main source of vegetable protein for the Guatemalan. However, this pulse is affected by anthracnose, a disease caused by the pathogen Colletotrichum lindemuthianum; this fungus can affect grain yield by up to 100%. The Institute of Agricultural Science and Technology (ICTA) has germplasm of bush-type beans in the highland region of the country; it has shown resistance to the disease under natural conditions. However, it was not evaluated using races of C. lindemuthianum reported before. The dry bean breeding program of Guatemala needs to identify resistant sources to the pathogen reported in the region; this is possible whenever a diverse germplasm is available. This research identified resistant sources to anthracnose using the inoculum of races 585 and 3981 of the pathogen; it was sprayed onto 216 accessions of the bush-type bean. Ten percent of the accessions were resistant (scale of 1-3) to both races of the pathogen, and the severity was measured using a scale of 1 to 9. Resistant sources found for C. lindemuthianum can be useful in the dry bean breeding program in Guatemala or worldwide.

Downloads

Download data is not yet available.

Author Biographies

Carlos Rául Maldonado-Mota, Instituto de Ciencia y Tecnología Agrícolas

Programa de mejoramiento de frijol común ICTA

Fitomejorador

María Gabriela Tobar Piñón, Instituto de Ciencia y Tecnología Agrícolas

Disciplina de biotecnología, ICTA

Coordinadora

Julio Cesar Villatoro Mérida, Instituto de Ciencia y Tecnología Agrícolas

Instituto de Ciencia y Tecnología Agrícolas

Gerente general

References

Akibode, C. S., & Maredia M. K. (2012, October 12). Global and regional trends in production,

trade and consumption of food legume crops (Staff Paper No. 2012-10). Michigan State University, Department of Agricultural, Food and Resource Economics. https://doi.org/10.22004/ag.econ.136293

Alzate-Marin, A. L., Costa, M. R., Arruda, K. M., De Barros, E. G., & Moreira, M. A. (2003). Characterization of the anthracnose resistance gene present in Ouro Negro (Honduras 35) common bean cultivar. Euphytica, 133(2), 165-169. https://doi.org/10.1023/A:1025551006444

Assefa, T., Mahama, A. A., Brown, A. V., Cannon, E. K., Rubyogo, J. C., Rao, I. M., Blair, M. W. & Cannon, S. B. (2019). A review of breeding objectives, genomic resources, and marker-assisted methods in common bean (Phaseolus vulgaris L.). Molecular Breeding, 39(2), 20. https://doi.org/10.1007/s11032-018-0920-0

Balardin, R. S., & Kelly, J. D. (1998). Interaction between Colletotrichum lindemuthianum racesand gene pool diversity in Phaseolus vulgaris. Journal of the American Society for Horticultural Science, 123(6), 1038-1047. https://doi.org/10.21273/JASHS.123.6.1038

Bastidas, O. (2017). Cell counting with Neubauer Chamber basic hemocytometer usage (Technical note). https://mural.uv.es/basgaros/Cell-counting-Neubauer-chamber.pdf

Beebe, S., Skroch, P. W., Tohme, J., Duque, M. C., Pedraza, F., & Nienhuis, J. (2000). Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Science, 40(1), 264-273. https://doi.org/10.2135/cropsci2000.401264x

Beninger, C. W., Hosfield, G. L., Bassett, M. J., & Owens, S. (2000). Chemical and morphological expression of the B and Asp seedcoat genes in Phaseolus vulgaris. Journal of the American Society for Horticultural Science, 125(1), 52-58. https://doi.org/10.21273/JASHS.125.1.52

Broughton, W. J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., & Vanderleyden, J. (2003). Beans (Phaseolus spp.) –model food legumes. Plant and Soil, 252(1), 55-128. https://doi.org/10.1023/A:1024146710611

Castellanos, G., Jara, C., & Mosquera, G. (2016). Bean pathogens: Practical guide for lab and greenhouse work (2a. ed.). Centro Internacional de Agricultura Tropical.

Cojulún, R. (1976). Libro de campo evaluación de germoplasma de frijol arbustivo y voluble. Instituto de Ciencia y Tecnología Agrícolas.

Fernández, O., Fernando, O., Gepts, P. L., López Genes M., & Arregocés, O. (1986). Stages of development of the common bean plant. Centro Internacional de Agricultura Tropical.

Ferreira, J. J., Campa, A., & Kelly, J. D. (2013). Organization of genes conferring resistance to anthracnose in common bean. En R. Varshney & R. Tuberosa (Eds.), Translational Genomics for Crop Breeding, Vol 1: biotic stress (pp.151-181). Wiley-Blackwell.

Freyre, R., Skroch, P. W., Geffroy, V., Adam-Blondon, A. F., Shirmohamadali, A., Johnson, W. C., Llaca, V., Nodari, R. O., Pereira, P. A., Tsai, S.-M., Tohme, J., Dron, M., Nienhuis, J., Vallejos, C. E. & Gepts, P. (1998). Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theoretical and Applied Genetics, 97(5-6), 847-856. https://doi.org/10.1007/s001220050964

Gepts, P. (1988). A Middle American and an Andean common bean gene pool. En Genetic resources of Phaseolus beans (pp. 375-390). Springer Dordrecht. https://doi.org/10.1007/978-94-009-2786-5

González, A. M., Yuste-Lisbona, F. J., Rodiño, A. P., De Ron, A. M., Capel, C., García-Alcázar, M., Lozano, R., & Santalla, M. (2015). Uncovering the genetic architecture of Colletotrichum lindemuthianum resistance through QTL mapping and epistatic interaction analysis in common bean. Frontiers in Plant Science, 6, 141. https://doi.org/10.3389/fpls.2015.00141

Gonçalves-Vidigal, M. C., Vallejo, V., & Kelly, J. D. (2003). Characterization of the anthracnose resistance in the differential cultivar Widusa. En Bean Improvement. Cooperative (No. 46).

Gragnolati, M., & Marini, A. (2003). Malnutrition and poverty in Guatemala (Vol. 2967). World Bank Publications.

Kelly, J. D., & Vallejo, V. A. (2004). A comprehensive review of the major genes conditioning resistance to anthracnose in common bean. HortScience, 39(6), 1196-1207. https://doi.org/10.21273/HORTSCI.39.6.1196

Legume Innovation Lab. 2014. Report Feed the Future Innovation Lab for Collaborative

Research on Grain Legumes: Fiscal Year 2014 Report. United States Agency for International Development. http://pdf.usaid.gov/pdf_docs/PA00KR44.pdf

Maldonado Mota, C. R. (2017). Identification of new sources of resistance to anthracnose in climbing bean germplasm from Guatemala [Tesis de maestria, North Dakota State University]. https://hdl.handle.net/10365/28547

Markell, S., Wunsh, M., & del Rio, L. (2012). Anthracnose of dry beans. NDSU Extension Service. North Dakota Agricultural Experiment Station.

McClean, P. E., Lee, R. K., Otto, C., Gepts, P., & Bassett, M. J. (2002). Molecular and

phenotypic mapping of genes controlling seed coat pattern and color in common bean (Phaseolus vulgaris L.). Journal of Heredity, 93(2), 148-152. https://doi.org/10.1093/jhered/93.2.148

Melotto, M., & Kelly, J. D. (2000). An allelic series at the Co-1 locus conditioning resistance to anthracnose in common bean of Andean origin. Euphytica, 116(2), 143-149. https://doi.org/10.1023/A:1004005001049

Mendoza, A., Hernández, F., Hernández, S., Ruíz, D., de la Vega, O. M., Mora, G., Acosta, J.,

& Simpson, J. (2001). Identification of Co-1 anthracnose resistance and linked molecular markers in common bean line A193. Plant Disease, 85(3), 252-255. https://doi.org/10.1094/PDIS.2001.85.3.252

Osorno, J. M. (2015). Genetic improvement of Middle-American climbing beans in Guatemala

(SO1.A1). Feed the Future, Legume Innovation Lab. https://www.canr.msu.edu/legumelab/uploads/files/SO1.A1-FY2015_Annual_Technical_Progress_Report_Leg_Innovation_Lab.pdf

Pastor-Corrales, M. A. (1991). Estandarización de variedades diferenciales y de designación de razas de Colletotrichum lindemuthianum. Phytopathology, 81(6), 694.

Pastor-Corrales, M. A. (1996). Traditional and molecular confirmation of the coevolution of

beans and pathogens in Latin America. Bean Improvement Cooperative,

, 46-47.

Pastor-Corrales, M. A., Erazo, O. A., Estrada, E. I., & Singh S. P. (1994). Inheritance of anthracnose resistance in common bean accession G 2333. Plant Disease, 78(10), 959-961. https://doi.org/10.1094/PD-78-0959

Pastor-Corrales, M. A., Otoya, M. M., & Maya, M. M. (1993). Diversidad de la virulencia de Colletotrichum lindemuthianum en Mesoamérica y la Región Andina. Fitopatología, 17(1-2), 31-38.

Schwartz, H. F., Corrales, M. P., & Singh, S. P. (1982). New sources of resistance to anthracnose and angular leaf spot of beans (Phaseolus vulgaris L.). Euphytica, 31(3), 741-754. https://doi.org/10.1007/BF00039213

Singh, S. P., Gepts, P., & Debouck, D. G. (1991). Races of common bean (Phaseolus

vulgaris, Fabaceae). Economic Botany, 45(3), 379-396. https://doi.org/10.1007/BF02887079

van Schoonhoven, A., & Pastor-Corrales, M. A. (1987). Standard system for the evaluation of bean germplasm. Centro Internacional de Agricultura Tropical.

Vallejo, V. A., Awale, H. E., & Kelly, J. D. (2003). Characterization of the anthracnose resistance in the Andean bean cultivar Jalo EEP558. Bean Improvement Cooperative, 44,179-180.