Cristalogénesis biológica y cristalografía en la elucidación de la estructura tridimensional de las proteínas. Revisión narrativa


  • Omar E. Vélasquez-González Instituto de Química, Universidad Nacional Autónoma de México y Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala
  • Luis Amézquita-Morataya Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala


Palabras clave:

Estructura tridimensional de las proteínas, cristalogénesis biológica, cristalografía, difracción de rayos X, diseño de fármacos


La obtención de información estructural tridimensional de una proteína es de suma importancia en campos tan variados como la bioquímica funcional, las ciencias de materiales o biomédicas. Siendo actualmente la difracción de rayos X de monocristal el estándar de oro para la consecución de este objetivo, la obtención de dicho monocristal sigue siendo un cuello de botella desde el punto de vista práctico, y poco entendido desde el punto de vista teórico. En este artículo se revisa desde la perspectiva estructural de la proteína la forma en que los rayos X permiten obtener la información estructural y las condiciones fisicoquímicas que permiten la formación de un cristal adecuado para estos experimentos.


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Biografía del autor/a

Omar E. Vélasquez-González, Instituto de Química, Universidad Nacional Autónoma de México y Unidad de Química Teórica y Computacional, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos, Guatemala

Licenciado en Química USAC, Maestro en Ciencias Químicas UNAM, Profesor Titular Departamento de Fisicoquímica, Facultad de Farmacia, USAC. 


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Cómo citar

Vélasquez-González, O. E., & Amézquita-Morataya, L. (2022). Cristalogénesis biológica y cristalografía en la elucidación de la estructura tridimensional de las proteínas. Revisión narrativa. Ciencia, Tecnologí­a Y Salud, 9(2), 199–214.



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